Male Bally G/Strain mice weighing approximately 20gm. were utilized in this study.
The cycloheximide in a concentration of 0.4 mg. per ml. , freshly prepared. in 0,9% saline, was administered intraperitoneally as a single 0.2mg./110g body weight of cycloheximide.
The control animals received a corresponding volume of. saline by same route.
Following the cycloheximide injection, 3 pairs each were sacrificed on days 1, 3, 5, 7 and 10.
Thirty, minutes prior to sacrifice. 4μ/gm. body weight of leucine C^(14)(Specific Activity 278mc/mM) were injected.
Piece of sublingual gland from each animal were fixed in paraformaldehyde in cacodylate buffer and double embedded in parlodion paraffin and sectioned of 4μ and stained with tohidme blue O.
The present study was undertaken to determine the specific effects of cycloheximide on the components of the pathways of protein synthesis in rat sublingual gland and relative dosage of drug to its relative effect on protein synthesis, RNA synthesis, and DNA synthesis.
The following charies were seen:.
1. After adminstration of cycloheximide, the weight of sublingual gland were increased compared with control.
2. Following injection of the cycloheximide to mice for 5 days ´cytologically, many the acinar cells are enlarged, lack the normal basophilia and show a marked accumulation of zymogen granules which becomes dispersed throught the cell.
3. The cycloheximide markedly affected the incorporation of leucine-C^(14) into mice sublingual gland protein through day 5 after which recovery was noted as shown by the return of incorporation rates to approach that of control.
4. These studies indicate as a follows;
First, protein synthesis is rapidly inhibited, as evidenced by decreased incorporation of leucine C^(14) into sublingual gland protein.
This primary effect is probably due to an interference with the formation of the peptide chain.
Secondly, the DNA-polymerase content is markedly affected in the sublingual gland cells of drug treated mice.
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